Figure 4.
Figure 4. Dock7 is required for migration and the activation of Rac1, Cdc42, and JNK induced by NRG1 in Schwann cells. (A and B) Schwann cells were transfected with control, Dock7-1, or Dock7-2 siRNA and incubated with or without NRG1 in Boyden chambers (12 independent fields). To confirm the effects of siRNA, the lysates of transfected cells were immunoblotted with an anti-Dock7, Rac1, Cdc42, or actin antibody. (C and D) Schwann cells were transfected with control or Dock7-1 siRNA and stimulated with NRG1 for 60 min. The activities of Rac1 and Cdc42 were assayed by affinity precipitation with GST-Pak1-CRIB (n = 3). (E) Cells were transfected with control or Dock7-1 siRNA and JNK phosphorylation was measured (n = 3). Error bars show ±SD. Data were evaluated by using one-way ANOVA (*, P < 0.01).

Dock7 is required for migration and the activation of Rac1, Cdc42, and JNK induced by NRG1 in Schwann cells. (A and B) Schwann cells were transfected with control, Dock7-1, or Dock7-2 siRNA and incubated with or without NRG1 in Boyden chambers (12 independent fields). To confirm the effects of siRNA, the lysates of transfected cells were immunoblotted with an anti-Dock7, Rac1, Cdc42, or actin antibody. (C and D) Schwann cells were transfected with control or Dock7-1 siRNA and stimulated with NRG1 for 60 min. The activities of Rac1 and Cdc42 were assayed by affinity precipitation with GST-Pak1-CRIB (n = 3). (E) Cells were transfected with control or Dock7-1 siRNA and JNK phosphorylation was measured (n = 3). Error bars show ±SD. Data were evaluated by using one-way ANOVA (*, P < 0.01).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal