Partial rescue of the perlecan morphants by human perlecan and endorepellin. (A) Coomassie blue–stained 3–8% Tris-borate gradient gel of human perlecan immunopurified from human coronary artery endothelial cells (left). Note the large and broad band corresponding to M_r of >500 kD. The right panel shows a Coomassie blue–stained 10% SDS-PAGE of human endorepellin. (B and C) Composite photographs of control, MO-DI, and MO-DI coinjected with perlecan or endorepellin at the designated concentrations. The rescue phenotype was calculated based on the degree of body twisting as compared with the microinjection of MO-DI alone. Notice the partial rescue of the twisted body phenotype in both cases. Bar, 500 μm.