Clp1 at the CR regulates cytokinesis. (A) Cells of the indicated genotype were assayed for colony formation on YE plates after 3 d at 32°C. (B and C) Cells of the indicated genotype were grown to mid log phase, shifted to 32°C for 5 h, ethanol fixed, and then stained with methyl blue and DAPI. At least 200 cells were scored for number of nuclei. Bar, 5 μm.