Figure 4. Clp1 regulates myosin II... | Rockefeller University Press

Figure 4.

$Figure 4. Clp1 regulates myosin II dynamics at the CR. (A–C) Confocal FRAP measurements of GFP-Myo2 (A), Rlc1-GFP (B), and YFP-Rng2 (C) at the ring in wild-type, clp1Δ, clp1-C286S, and mid1Δ431-481 cells. Fluorescence recovery curves, including half-times with standard error, number of cells per strain analyzed, and mobile fractions are shown. Best-fit curves derived from the mean intensity values of n cells (Prism software) were used to calculate half-times and mobile fractions. R2 values range from 0.9552 to 0.9869. Asterisks indicate significant differences between wild-type and experimental cells as determined by student's t test. P-values and standard error are included.$

Clp1 regulates myosin II dynamics at the CR. (A–C) Confocal FRAP measurements of GFP-Myo2 (A), Rlc1-GFP (B), and YFP-Rng2 (C) at the ring in wild-type, clp1Δ, clp1-C286S, and mid1Δ_431-481 cells. Fluorescence recovery curves, including half-times with standard error, number of cells per strain analyzed, and mobile fractions are shown. Best-fit curves derived from the mean intensity values of n cells (Prism software) were used to calculate half-times and mobile fractions. R² values range from 0.9552 to 0.9869. Asterisks indicate significant differences between wild-type and experimental cells as determined by student's t test. P-values and standard error are included.