Correlative live cell imaging and electron microscopy of filopodia transitions. (A) Image of a cell transfected with EGFP-fascin (green) and mCherry-actin (red) after fixation at the end of the video sequence. (B) Electron micrograph of region shown in A after negative staining. Boxes and arrows in A and B indicate regions corresponding to the video sequences shown in C and D. Arrows in C and D indicate transition steps of filopodia into lamella, with the eventual depletion of fascin; final panels show actin label alone in the fixed cell. Times are given in minutes and seconds. See Video 5 .