Figure 3.

Increased exchange of α5-integrin and paxillin in FAs of Mgat5−/− cells. (A) Representative images of live Mgat5+/+, Mgat5−/−, and Rescue cells transfected with α5-integrin-GFP are presented. Quantification of α5-integrin-GFP fluorescence recovery after bleaching in FA (B) and non-FA (C) ROIs over time is presented for Mgat5+/+, Mgat5−/−, and Rescue cells. (D) Quantification of FA-associated paxillin-GFP fluorescence recovery over time is presented for Mgat5+/+, Mgat5−/−, and Rescue cells. The boxes show the percentage of recovery and, for paxillin, the half-time of recovery (*, P < 0.05).

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