Figure 2. Increased FAK exchange in FAs... | Rockefeller University Press

Figure 2.

$Figure 2. Increased FAK exchange in FAs of Mgat5−/− cells. (A) Mgat5+/+, Mgat5−/−, and Rescue cells transfected with FAK-GFP were imaged before laser bleaching of one FA-containing region of interest (ROI; red square). A time-lapse sequence (in seconds) shows the corresponding ROI before photobleaching (prebleach), immediately after photobleaching (bleach), and during recovery (recovery). Quantification of FAK-GFP fluorescence over time is presented for Mgat5+/+, Mgat5−/−, and Rescue cells in FA (B) or non-FA (C) ROIs. Percentage of recovery (boxes) shows the extent of FAK-GFP mobile fraction. *, P < 0.05. Bar, 20 μm.$

Increased FAK exchange in FAs of Mgat5^−/− cells. (A) Mgat5^+/+, Mgat5^−/−, and Rescue cells transfected with FAK-GFP were imaged before laser bleaching of one FA-containing region of interest (ROI; red square). A time-lapse sequence (in seconds) shows the corresponding ROI before photobleaching (prebleach), immediately after photobleaching (bleach), and during recovery (recovery). Quantification of FAK-GFP fluorescence over time is presented for Mgat5^+/+, Mgat5^−/−, and Rescue cells in FA (B) or non-FA (C) ROIs. Percentage of recovery (boxes) shows the extent of FAK-GFP mobile fraction. *, P < 0.05. Bar, 20 μm.