acr1-936 had both mitotic and interphase defects. (A) DAPI-stained wild-type, nuc1-632, and acr1-936 cells that were cultured at 36°C for 4–8 h. The insets correspond to the white dashed boxes in the left panels. (B) Localization of Nuc1 was dispersed in the nucleus in the acr1-936 mutant. Bars, 10 μm.