Actin polymerization is inhibited by WAVE2 siRNA but restored in N-WASP/WAVE2 double KD cells. (A) Representative images of actin polymerization were measured using the BE assay of cells (left columns) and total F-actin (right columns) treated with scrambled (control), WAVE2, N-WASP, N-WASP/WAVE2, N-WASP/WAVE2 + {YFP-DNmDia1 plasmid }, and Arp2/3 siRNA at 0 and 1 min after EGF stimulation, the time of maximum early generation of BE formation. Bar, 10 μm. (B) The relative number of BEs (arbitrary units of fluorescence intensity) at the leading edge in control, WAVE2, N-WASP, and N-WASP/WAVE2 is shown. Error bars are ±SEM of 80 cells from three independent experiments. (C) The relative number of BEs at the leading edge in control, N-WASP/WAVE2 siRNA with YFP-vector, and N-WASP/WAVE2 siRNA with YFP-DNmDia1 plasmid is shown. P-values are in comparison to control. Error bars are ±SEM of a total of 50 cells from at least three independent experiments. (D) The relative number of BEs at the leading edge in scrambled or Arp2/3 siRNA is shown. P-values are shown in comparison to control. Error bars are ±SEM of a total of 37 cells from three independent experiments.