WAVE2 is recruited to the leading edge of extending lamellipods after stimulation with EGF. (A) Cells were stimulated with EGF and stained with the C-14 anti-WAVE2 antibody. Bar, 10 μm. (B) Quantification of recruitment of WAVE2 in the leading edge. Mean fluorescent intensity of C-14 staining was measured at the leading edge and plotted versus time of EGF stimulation. (C) Ratio of WAVE2 fluorescent intensity at the leading edge to total cellular WAVE2 fluorescent intensity. Error bars are ±SEM of a total of 26 cells from three independent experiments.