Cindr localizes to and regulates F-actin. (A, B, D, and E) Canton S retinae dissected at times as indicated. Tissue was incubated in phalloidin to detect F-actin (white). Insets show detection of Cindr (green), DE-Cad (blue), and F-actin (red) in selected fields. (C and F) GMR>cindr-IR2+3 retina; phalloidin is shown. (G) High-magnification image of a single protruding ommatidial bristle at 41 h APF. The retina was torn before imaging to remove tissue below the bristle (top right). Cindr (green) is concentrated at the tip of the protruding bristle. Phalloidin (red) and DE-Cad (blue) are also shown. (H) Dorsal thorax of adult Canton S fly. (I) apterous>cindr IR2+3 adult thorax. Macrochaetea are missing, hooked, thickened, or stunted (arrows). (J) 28-h-APF pupal eye mosaic for cpascrd1. The lack of nuclear GFP labels mispatterned mutant cells. DE-Cad (blue, J′) was discontinuous (arrows) and phalloidin (red, J″) detects extremely elevated levels of polymerized actin. Bars: (A–G and J) 5 μm; (H and I) 100 μm.