Deregulation of DE-Cad and Rst distribution when Cindr is reduced. (A, C, and E) GMR>cindr-IR²⁺³ retina processed to detect DE-Cad (green in overlay panel) and Rst (red) dissected at 25, 30, and 41 h APF. DE-Cad was irregularly distributed (green arrows). Rst remains strongly localized to IPC–IPC membranes (red arrows) and, by 41 h APF, was cytoplasmic in IPCs except for a subset that have large apical profiles resembling those of 1° cells (asterisks). These cells may have temporarily contacted cone cells earlier and received 1°-inductive signals (Miller and Cagan, 1998). (B, D, and F) Control GMR>lacZ ommatidia showing wild-type distribution of DE-Cad and Rst. Bars 10 μm.