PLK-1 and CDC-25.1 levels in par mutants. Normalized fluorescent intensities in AB and P1 for cytoplasmic PLK-1 (A) and nuclear CDC-25.1 (B) immunostained wild-type, par-1, par-2(RNAi), par-3, par-4, and mex-5/mex-6(RNAi) two-cell embryos. Normalized means ± SEM shown. Corresponding p-values are given in Table S2 (C and D).