ARF6 and paxillin fail to colocalize during early fusion in Brag2-deficient cells. (A) Representative images of CntSi, (B) Brag2Si, and (C) Dock180Si myoblasts after d 3 in DM depicted in phase contrast and immunofluorescence, labeled for ARF6 (green), paxillin (red), and merged with Hoechst 33258 (blue). Arrowheads point to clusters of paxillin observed in Brag2 and to a lesser extent in Dock180 knockdowns. Arrows point to colocalization sites of ARF6 and paxillin in the merged fields. Bar, 50 μm.