Brag2-deficient myoblasts exhibit aberrant paxillin localization during differentiation. (A) Representative images of CntSi and Brag2Si myoblasts after d 3 in DM depicted in phase contrast and immunofluorescence, labeled with Hoechst 33258 (blue), myogenin (red), paxillin (yellow), and merged. Bar, 50 μm. (B) Paxillin redistribution in myogenin-positive cells, d 3 in DM. At least 1,000 myogenin-positive nuclei were counted for each experimental trial, and the error bars indicate the mean ± SE of three independent determinations (*, P < 0.01). (C) Western blot analysis of paxillin expression after d 5 in DM under fusion conditions. Paxillin migrates as a 65-kD protein.