Dock180 deficiency in myotubes increases cellular levels of β₁-integrin. (A) Western blot analysis of total protein levels of β₁-integrin at d 6 in DM. β₁-integrin migrates as a 130-kD protein and loading control GAPDH migrates as 35-kD protein. (B) Western blot analysis of total protein levels of E-cadherin. E-cadherin migrates as a 120-kD protein; the 90-kD band likely reflects digestion of the extracellular domain during lysate preparation. (C) Western blot analysis of M-cadherin total protein levels. M-cadherin migrates as a 130-kD protein.