Figure 1.
Figure 1. Expression and knockdown of Brag2 and Dock180 in C2C12 myoblasts. (A) Brag2 (1,100 bp) and Dock180 (2,485 bp) expression during growth (GM) and differentiation (DM) as shown by RT-PCR. GAPDH (250 bp). (B) Total RNA from cell lines Brag2Si, Dock180Si, and DKD, was harvested after d 4 in DM, and tested for Brag2 and Dock180 expression by RT-PCR. (C) Quantitative PCR analysis of Brag2 (dark gray) and Dock180 (light gray) expression in GM and d 4 DM. Values are normalized to the expression in CntSi cells (white). (D) Quantitative PCR analysis of Brag2 and Dock180 expression from Brag2Si, Dock180Si, and DKD cells. Values are normalized to the expression of CntSi and Cnt2Si cells. Error bars indicate the mean ± SE of three independent determinations.

Expression and knockdown of Brag2 and Dock180 in C2C12 myoblasts. (A) Brag2 (1,100 bp) and Dock180 (2,485 bp) expression during growth (GM) and differentiation (DM) as shown by RT-PCR. GAPDH (250 bp). (B) Total RNA from cell lines Brag2Si, Dock180Si, and DKD, was harvested after d 4 in DM, and tested for Brag2 and Dock180 expression by RT-PCR. (C) Quantitative PCR analysis of Brag2 (dark gray) and Dock180 (light gray) expression in GM and d 4 DM. Values are normalized to the expression in CntSi cells (white). (D) Quantitative PCR analysis of Brag2 and Dock180 expression from Brag2Si, Dock180Si, and DKD cells. Values are normalized to the expression of CntSi and Cnt2Si cells. Error bars indicate the mean ± SE of three independent determinations.

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