PKD-2∷GFP localizes to abnormally formed CEM cilia in nphp-1 and nphp-4 mutants. 3D deconvolution images of a single CEM cilium in wild type (a–c), nphp-1 (d–f), and nphp-4 (g–i). See also Videos 1–3 . Cilium structure is labeled by β-tubulin (Ppkd-2∷tbb-4∷tdTomato; c, f, and i). PKD-2∷GFP localizes to the TZ and cilium proper in the wild type (a), to abnormally curled nphp-1 cilia (d), and to nphp-4 stunted cilia (g). Drawings show relationship between reporters in context of the male nose. Bar, 5 μm.