Summary of NPC disassembly and reassembly kinetics. (A and B) Overview over all means of disassembly (A) and assembly (B) kinetics. (C) Time points of first visible nuclear accumulation over background for all analyzed Nups. (D) Time points of 50% assembly of Nups relative to the first derivative of IBB intensity as a measure for import rate. Because of the change in concentration distribution of IBB between cytoplasm and nucleus during the import phase, the first derivative of IBB intensity systematically underestimates true instantaneous import rates. The maximum reached at time point 0 therefore does not reflect the true maximal import rates, which may be reached later. (E and F) Models for mitotic NPC disassembly and reassembly. Filament structures are included in the model in gray on the basis of previous data. The precise positions of the Nups in the NPC are unknown and thus drawn schematically. Because the different Nup-expressing cell lines showed some variability in the timing of mitotic progression (10.6 ± 1.5 min from anaphase onset to t_1/2[import]; not depicted), the time between anaphase onset and t_1/2(import) was normalized to 10 min in B to D. Error bars indicate SD.