Time series representing the dissociation of four Nups from the NE during prophase. The contrast of the image series was normalized to a common maximal mean intensity on the nuclear rim at the first time point of each series. Plots show the data obtained from the series shown (red) and the mean of n series (black). As a reference, mean intensities of Nup98 (cyan) and POM121 (green) are shown in all plots. Time stamps give min:s relative to t_1/2(import). Videos 3 and 4 show representative full-image sequences for Nup98 and POM121. Error bars indicate SD.