Localization pattern of Nups on chromatin during anaphase. Cells were followed from metaphase and single images were taken at defined time points. Images were filtered with an anisotropic diffusion filter. Boxes indicate regions of enlargements. Intensity profiles measured along a 0.45-μm-wide line as indicated by the white outlines were plotted after subtraction of cytoplasmic background. Time stamps indicate minutes after anaphase onset. (A) Cells expressing GFP-tagged Nup107, Nup133, and Nup37. (B) Cells expressing GFP-Nup107, GFP-Nup133, and POM121-mCherry. (C) Cells expressing GFP- Nup107, GFP-Nup133, and LBR-mCherry.