Quantification of Nup and import marker fluorescence intensities. (A and B) Regions of interest (outlines) were obtained from the Hoechst channel automatically (whole nucleus) or interactively (NE) and mean intensities were measured in the IBB and Nup channels. Time stamps give min:s relative to t_1/2(import). (C and D) Normalized Nup intensities over time extracted from sequences shown in A and B after alignment to t_1/2(import) (green). Black curves represent the mean of five independent experiments (error bars indicate SD). Red curves, IBB mean.