Chromosome segregation in human tumor cell lines.GFP–histone H2B was expressed in colon carcinoma HCT116 and Caco2 cells and in breast cancer MCF-7 cells. Images selected from time-lapse videos are shown with times given in minutes. Arrowheads identify unaligned chromosomes in prometaphase, and arrows identify lagging chromatids at anaphase. Bars, 5 μm.