Unregulated moesin phosphorylation affects organization of the cell cortex and microtubule spindle. (A) Selected frames of dividing α-tubulin–GFP cells expressing moesin-T559A or -T559D. The red lines show maximal deviations of the spindle orientation from its initial position (green lines). (B) Relative proportions of mitotic cells displaying cortical defects (left) and destabilization of the microtubule orientation (right) after depletion in moesin or Slik and after expression of the moesin-T559A or -T559D phosphomutant moesin forms. Error bars represent SD. Bars, 10 μm.