Figure 3.
Figure 3. Analyzing GFP–CLIP-170 on MT ends with FCS. (A) Confocal image of a COS-7 cell transiently transfected with GFP–CLIP-170. The plus sign indicates the location of FCS measurement. Bar, 5 μm. (B) Intensity track of FCS measurement in a transfected COS-7 cell. Peaks of fluorescence are occasionally detected. The dotted red line indicates the exponential fluorescence decay; the bottom double-headed arrows indicate cytoplasmic fluorescence; the top double-headed arrows indicate peak fluorescence. (C) Comparison of the number of cytoplasmic and peak-bound GFP–CLIP-170 particles. Values as depicted in B were measured, and the number of particles was determined for 110 peaks. A scatter plot of these values is best approximated by the curve (indicated by the red line) Y = Ymax × (1 − e−kx).

Analyzing GFP–CLIP-170 on MT ends with FCS. (A) Confocal image of a COS-7 cell transiently transfected with GFP–CLIP-170. The plus sign indicates the location of FCS measurement. Bar, 5 μm. (B) Intensity track of FCS measurement in a transfected COS-7 cell. Peaks of fluorescence are occasionally detected. The dotted red line indicates the exponential fluorescence decay; the bottom double-headed arrows indicate cytoplasmic fluorescence; the top double-headed arrows indicate peak fluorescence. (C) Comparison of the number of cytoplasmic and peak-bound GFP–CLIP-170 particles. Values as depicted in B were measured, and the number of particles was determined for 110 peaks. A scatter plot of these values is best approximated by the curve (indicated by the red line) Y = Ymax × (1 − e−kx).

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