CBF is reduced in hydin mutants. Images from videos (a and c), line scans (b and d), and corresponding plots (e and f) of ependymal cilia from wild-type (a, b, and e) and mutant animals (c, d, and f). The position of the line used for preparing the line scan is indicated in panels a and c (arrowhead and white line); the part of the line scan used for preparing the plots is indicated in b and d (arrow). (g) CBF for wild-type (open squares) and mutant (closed squares) animals based on the plots shown in e and f. (h) CBF of tracheal cilia in samples from a hydrocephalic OVE459 mutant (closed squares) and a wild-type littermate (open squares).