Centrosome separation is inhibited after inactivation of Akt or APC2. (A) Live confocal analysis of embryos expressing α-tubulin–GFP. Examples of the angle of centrosome separation 20 s before NEB in wild-type (WT) embryos, akt1^q/akt1⁰⁴²²⁶ embryos (akt), embryos injected with anti-Akt antibodies (α-Akt), and embryos expressing an allele of APC2 unable to bind to Arm (apc2Δs). Bar, 10 μm. (B) Histogram representing the mean angle of centrosome separation before NEB in embryos. Data for each class was obtained from 100 individual nuclei. Errors bars indicate the SEM. See Videos 4–8 .