Figure 2.
Figure 2. SEPT2 is required for efficient Golgi-to-plasma membrane transport of basolateral and apical membrane proteins. (A and B) Preimmune (mock; blue) or anti-SEPT2 (red) IgGs were microinjected into MDCK cells with plasmid vectors encoding for the temperature-sensitive mutant of the basolateral membrane protein VSV-G (ts045-VSV-G-YFP; A) or the apical protein p75-GFP (B). After accumulation of tsO45-VSV-G-YFP and p75-GFP in the Golgi complex at 19°C, live cells were imaged at 32°C. Arrows point to the plasma membrane of cell–cell contacts; arrowheads point to juxtanuclear Golgi regions. Bars, ∼5 μm. (C and D) Net fluorescence intensities of cell surface (plasma membrane) and juxtanuclear Golgi regions were measured. Scatter plots show ratios (mean values ± SEM; n = 6) of plasma membrane to Golgi fluorescence for ts045-VSV-G-YFP– (C) and p75-GFP–expressing cells (D). Data are representative of three different experiments.

SEPT2 is required for efficient Golgi-to-plasma membrane transport of basolateral and apical membrane proteins. (A and B) Preimmune (mock; blue) or anti-SEPT2 (red) IgGs were microinjected into MDCK cells with plasmid vectors encoding for the temperature-sensitive mutant of the basolateral membrane protein VSV-G (ts045-VSV-G-YFP; A) or the apical protein p75-GFP (B). After accumulation of tsO45-VSV-G-YFP and p75-GFP in the Golgi complex at 19°C, live cells were imaged at 32°C. Arrows point to the plasma membrane of cell–cell contacts; arrowheads point to juxtanuclear Golgi regions. Bars, ∼5 μm. (C and D) Net fluorescence intensities of cell surface (plasma membrane) and juxtanuclear Golgi regions were measured. Scatter plots show ratios (mean values ± SEM; n = 6) of plasma membrane to Golgi fluorescence for ts045-VSV-G-YFP– (C) and p75-GFP–expressing cells (D). Data are representative of three different experiments.

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