Anillin recruits septin to the cleavage furrow and to filamentous structures in LatA. (A–C) Nontransfected (A and B) and anillin-GFP–expressing (C) S2 cells fixed during anaphase/telophase and labeled with antibodies to septin^Pnut (middle; red in merged) and anillin (A and B, left; green in merged) and the DNA stain HOECHST (blue in merged; maximum intensity projections of deconvolved 0.25-μm sections). C' is a 90° rotation about the y axis of the projected stack within the boxed region in C. (D) Time-lapse sequence of a cell expressing anillin-GFP attempting cytokinesis in the presence of LatA after 6 d of septin^Pnut RNAi. (E–G) Nontransfected S2 cells fixed during anaphase/telophase and labeled with antibodies to septin^Pnut (middle; red in merged) and Dia (left; green in merged) and the DNA stain HOECHST (blue in merged; maximum intensity projections of deconvolved 0.25-μm sections). (E) Control cell. (F) Cell after 3 d of anillin RNAi. (G) Cell after 3 d of anillin RNAi and LatA treatment combined. Bars, 5 μm.