Myosin II localization can occur independently of F-actin and anillin but stable furrow positioning requires anillin. (A and B) Frames from time-lapse sequences of cells expressing MRLC^Sqh-GFP progressing through anaphase. (A) Control cell expressing MRLC^Sqh-GFP. (B) Cell treated with LatA. A projection of five 2-μm sections is shown (Video 6, available). (C and D) Cells expressing anillin-GFP (green in merged) fixed during anaphase/telophase in the presence of 1 μg/ml LatA, labeled with a Ser21 phospho-MRLC^Sqh antibody (red in merged; maximum intensity projections of multiple 0.25-μm deconvolved sections). (E) Frames from a time-lapse sequence of a MRLC^Sqh-GFP cell attempting cytokinesis after 3 d of anillin RNAi (see Video 7). (F) Cell expressing MRLC^Sqh-GFP (green in merged), fixed during anaphase in the presence of LatA after anillin RNAi and labeled with an anillin antibody (red in merged; maximum intensity projection of multiple 0.25-μm deconvolved sections). (G) Frames from a time-lapse sequence of a cell expressing MRLC^Sqh-GFP attempting cytokinesis after 3 d of anillin RNAi and in the presence of LatA (projection of five 2-μm sections; see Video 8). Times are h:min:s from anaphase onset. Bars, 5 μm.