unc-108(n3263) mutants are defective in the incorporation of lysosomes to phagosomes. (A–C) Time-lapse recording of phagosomes containing C2 in wild-type (A) and unc-108(n3263) (B and C) embryos coexpressing Pced-12× FYVE::mrfp1 and Pced-1 ctns-1::gfp. Bars, 1 μm. (D) Summary of the formation of CTNS-1::GFP circles on phagosomal surfaces over time. Formation time, the interval between the appearance of a 2× FYVE::mRFP1 circle and a CTNS-1::GFP circle on the same phagosome. Data for wild-type embryos are presented as mean ± SD (n = 3). Data for unc-108(n3263) embryos are individually listed, as the CTNS-1::GFP puncta on most phagosomes failed to convert to continuous circles within the recording period. (E) Number of DIC(+) cell corpses (black) and CTNS-1::GFP(+) phagosomes (green) in twofold embryos. Results are reported as mean ± SD (n = 15).
