RT-PCR revealed that the endogenous RSP16 messages were transcribed and cleaved. (A) The schematic depicts the location of the primers (arrows) and products from the RT-PCR. (B) 3′ UTR was amplified from wild type and 12E8. No products were present in the control lacking reverse transcription. (C) RT-PCR of the seventh exon from 12E8 was much less robust than the wild type. Full-length cDNA was amplified from wild-type reverse transcription but not from 12E8.