Figure 3. WSC is localized to nascent... | Rockefeller University Press

Figure 3.

$Figure 3. WSC is localized to nascent and mature WBs, and effect of deletion of dynamin-related proteins and FIS1. (A) WSC localizes over HEX assemblies in nascent WBs and surrounds HEX assemblies in mature WBs. WSC–GFP and RFP-PTS1 were coexpressed and localized in apical and subapical compartments by laser-scanning confocal microscopy. Bar, 10 μm. Magnified views of the region are indicated by a black square and shown at the right. Arrowheads point to refractive HEX assemblies. (B) WSC–GFP is concentrated over HEX assemblies in nascent WBs. Individual images of a single nascent WB are shown on the left and GFP and RFP fluorescence intensity are shown on the right across the indicated line. A black bar in the graph indicates the position of the HEX assembly, which can be seen in the DIC image. The line in the merge panel is 5.25 μm. (C) Effect of deletion of fis1, vps1, and dnm1 on WB assembly. DIC images show refractive HEX assemblies at the cell cortex (arrowheads) and RFP-PTS1 reveals the peroxisomal matrix. Bar, 10 μm. Bottom shows peroxisomes in the apical compartment, where Δdnm1 and Δfis1 present abnormal tubular peroxisomes. Bar, 1 μm. Radial growth for the indicated strains is shown in μm/min.$

WSC is localized to nascent and mature WBs, and effect of deletion of dynamin-related proteins and FIS1. (A) WSC localizes over HEX assemblies in nascent WBs and surrounds HEX assemblies in mature WBs. WSC–GFP and RFP-PTS1 were coexpressed and localized in apical and subapical compartments by laser-scanning confocal microscopy. Bar, 10 μm. Magnified views of the region are indicated by a black square and shown at the right. Arrowheads point to refractive HEX assemblies. (B) WSC–GFP is concentrated over HEX assemblies in nascent WBs. Individual images of a single nascent WB are shown on the left and GFP and RFP fluorescence intensity are shown on the right across the indicated line. A black bar in the graph indicates the position of the HEX assembly, which can be seen in the DIC image. The line in the merge panel is 5.25 μm. (C) Effect of deletion of fis1, vps1, and dnm1 on WB assembly. DIC images show refractive HEX assemblies at the cell cortex (arrowheads) and RFP-PTS1 reveals the peroxisomal matrix. Bar, 10 μm. Bottom shows peroxisomes in the apical compartment, where Δdnm1 and Δfis1 present abnormal tubular peroxisomes. Bar, 1 μm. Radial growth for the indicated strains is shown in μm/min.