Figure 1. wsc mutants are defective in... | Rockefeller University Press

Figure 1.

$Figure 1. wsc mutants are defective in WB maturation and inheritance. (A) HEX assemblies move in concert with peroxisomes in wild-type hyphae (Video 1, available) but move randomly in the matrix of wsc mutant peroxisomes (Video 2). The peroxisome matrix is revealed by peroxisome-targeted GFP, and HEX assemblies are observed as characteristic refractive structures by light microscopy. Arrows point to peroxisomes containing HEX assemblies. Bar, 1 μm. A schematic of results obtained from time-lapse microscopy is presented below. The double-headed arrow indicates random motion of the HEX assembly. (B) HEX assemblies accumulate in the apical cell of the wsc mutant and are not observed in subapical compartments. Representative apical and subapical compartments of wild-type and wsc mutant hyphae viewed by light microscopy are shown. Arrows point to HEX assembly. Bar, 10 μm. (C) The top shows a quantification of HEX assembly distribution in wild-type and mutant hyphae. The bottom shows the distribution of nuclei at similar positions in the hypha. Standard deviation is indicated with error bars.$

wsc mutants are defective in WB maturation and inheritance. (A) HEX assemblies move in concert with peroxisomes in wild-type hyphae (Video 1) but move randomly in the matrix of wsc mutant peroxisomes (Video 2). The peroxisome matrix is revealed by peroxisome-targeted GFP, and HEX assemblies are observed as characteristic refractive structures by light microscopy. Arrows point to peroxisomes containing HEX assemblies. Bar, 1 μm. A schematic of results obtained from time-lapse microscopy is presented below. The double-headed arrow indicates random motion of the HEX assembly. (B) HEX assemblies accumulate in the apical cell of the wsc mutant and are not observed in subapical compartments. Representative apical and subapical compartments of wild-type and wsc mutant hyphae viewed by light microscopy are shown. Arrows point to HEX assembly. Bar, 10 μm. (C) The top shows a quantification of HEX assembly distribution in wild-type and mutant hyphae. The bottom shows the distribution of nuclei at similar positions in the hypha. Standard deviation is indicated with error bars.