Upon passage through mitosis, a targeted locus remains targeted in daughter cells. Targeting cells stably expressing histone H2A-YFP (green) were induced to express LacI-mCherry–Lamin B1 (red, arrow) and cells with a targeted locus were selected for imaging. Still images (a–r) are shown from time series (0–120 min, G2 to early G1 phase) of targeting cells stably expressing H2A-YFP progressing through mitosis. Images are projections of deconvolved z stacks taken every 20 min. Bar, 5 μm.