The targeted locus is stably anchored to the nuclear lamina during interphase. Targeting cells stably expressing histone H2A-YFP (green) were induced to express LacI-mCherry–Lamin B1 (red, arrow) and cells with a targeted locus were selected for imaging. Still images (a–o) are shown from an interphase time series (0–19 h, G1 to G2/M phase). Images are projections of deconvolved z stacks taken every 20 min. Bar, 5 μm.