Subcellular localization of GFP-Dsh in the axial mesoderm during C&E. (A–D) Confocal images of the axial mesoderm in live embryos expressing GFP-Dsh at the tailbud stage (10 hpf). (A′–D′) Confocal images of the same embryos as shown in A–D, but colabeled with memRFP. Histograms of the fluorescent intensity of the selected cells (*) are shown in the right panels. Green lines represent the fluorescent intensity of GFP-Dsh and the red lines represent the intensity of memRFP. (E, F, E′, and F′) GFP-Dsh localization in the axial mesoderm of the same WT embryo. Arrows show that GFP-Dsh was mainly localized in the cytoplasm at 75% epiboly (8 hpf) (E and E′), but became restricted to the posterior cell membrane at the tailbud stage (F and F′). (G) Quantification of GFP-Dsh localization in the axial mesoderm at the tailbud stage (Materials and methods). (A–F and A′–F′) Dorsal views. A, anterior; P, posterior. Bar, 20 μm.