Subcellular localization of GFP-Pk during C&E. (A, and D–G) Confocal images of the dorsal mesoderm in live embryos expressing GFP-Pk and membrane-RFP (memRFP) at the tailbud stage (10 hpf). Dashed lines illustrate the boundary between the axial and presomitic mesoderm. (B and C) GFP-Pk localization in the dorsal mesoderm of the same WT embryo at 8 hpf (B) and 10 hpf (C). (H) Quantification of GFP-Pk localization at the tailbud stage in WT and embryos deficient in noncanonical Wnt signaling (Materials and methods). (A–G) Dorsal views. A, anterior; P, posterior; Axial, axial mesoderm. Bar, 20 μm.