Computational modeling of cell intercalation behaviors during the medial PSM C&E. (A) Tissue shape changes predicted by the control simulation. Each blue block represents a single cell. (B) Quantitative comparison of the C&E rates calculated from the WT time-lapse analyses and the control simulations. Error bars represent the standard error. P > 0.3, simulated results vs. in vivo observation. (C–F) Comparison of the results from the control simulations and the simulations in which the cell behaviors were modified based on the kny;tri double-mutant data in one of the four ways: reducing the total amount of intercalations (C); increasing radial ML intercalation (D); reducing planar medial, radial AP, and coordinated radial/medial intercalations (E); and reducing planar medial, radial AP, and coordinated radial/medial intercalations, while increasing radial ML intercalation (F). 20 independent simulations were performed for the control and each modification. Error bars represent the standard error. A, anterior; M, medial. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.