Figure 2.
Actomyosin contractility is required for lip fusion. (A) Bilateral cleft lip (white arrows) is detected in E12.5 Myh9f/f; Myh10f/f; CrectTg/0 embryos lacking NMII function in the epithelium versus control, Myh9f/f; Myh10f/+; CrectTg/0, and Myh9f/+; Myh10f/f; CrectTg/0 embryos. Scale bar, 1 mm. Images were adjusted to gamma 1.3 to achieve more even brightness. (B) Time-lapse stills of F-actin in 9ts samples treated with DMSO (n = 3) or blebbistatin (n = 3). Treatment began 1 h prior to imaging. Yellow spheres and lines indicate distance measurement between MNP and LNP. MNP, medial nasal process; LNP, lateral nasal process. Time reads as hh:mm. Scale bars, 50 μm. (C) Blebbistatin-treated samples had less closure between nasal processes than DMSO-treated samples. Dots represent individual samples. Error bars represent the SEM. Data were compared using two-tailed Student’s t test.

Actomyosin contractility is required for lip fusion. (A) Bilateral cleft lip (white arrows) is detected in E12.5 Myh9f/f; Myh10f/f; CrectTg/0 embryos lacking NMII function in the epithelium versus control, Myh9f/f; Myh10f/+; CrectTg/0, and Myh9f/+; Myh10f/f; CrectTg/0 embryos. Scale bar, 1 mm. Images were adjusted to gamma 1.3 to achieve more even brightness. (B) Time-lapse stills of F-actin in 9ts samples treated with DMSO (n = 3) or blebbistatin (n = 3). Treatment began 1 h prior to imaging. Yellow spheres and lines indicate distance measurement between MNP and LNP. MNP, medial nasal process; LNP, lateral nasal process. Time reads as hh:mm. Scale bars, 50 μm. (C) Blebbistatin-treated samples had less closure between nasal processes than DMSO-treated samples. Dots represent individual samples. Error bars represent the SEM. Data were compared using two-tailed Student’s t test.

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