Endogenous DJ-1 suppressed cPGA modification in cell lysates. (A) Lysates of WT, DJ-1 KO, or DJ-1 KO SH-SY5Y cells expressing exogenous DJ-1 (WT or the indicated mutants) were treated with 1 mM cPGA and then phospho-glyceroyl modifications were detected by Phos-tag SDS-PAGE. The cells without exogenous DJ-1 expression are denoted as “none.” (B) WT or DJ-1 KO HeLa cells stably expressing pSu9-Halo-mGFP and Parkin were pulse labeled with Halo ligand, followed by treatment with antimycin A and oligomycin (AO) for the indicated times, and then immunoblotted. A Halo^ligand band (black arrowhead) appeared in a time-dependent manner in both WT and DJ-1 KO cells following AO treatment. The gray arrowhead indicates ∼40-kDa bands that are likely a cleavage product of the HaloTag construct generated during GFP chromophore formation. All blots are representative of at least two independent experiments. Source data are available for this figure: SourceData F8.