Abnormal nuclear morphology upon SPT inhibition arises following cell division. (a) Proliferation of RPE-1 cells in the medium with 10% FBS or LD-FBS without or with 5 µM myriocin for 48 h. Cell numbers were quantified with a Coulter counter. Error bars represent the standard deviation (n = 3 biological replicates). NS, P > 0.05. (b) Transcriptome profile of RPE-1 cell culture in 10% FBS or LD-FBS. The number of genes upregulated in LD-FBS compared with FBS media includes every enzyme in the cholesterol biosynthesis pathway. (c) Transcriptome profile of RPE-1 cells cultured in LD-FBS without and with 5 µM myriocin for 24 h. No significant enrichment of gene ontology terms found in the genes up- or downregulated. (d) Time lapse of live-cell microscopy images of RPE-1 expressing GFP-histone H2B in LD-FBS. Scale bar = 10 µm. (e) Time lapse of live-cell microscopy images of RPE-1 expressing GFP-histone H2B in LD-FBS in the presence of 5 µM myriocin for 20 h. Scale bar = 10 µm. (f) Quantification of the circularity of the nuclei of daughter cells 2 h after metaphase was observed in control cells or cells treated with 5 µM myriocin for 20 h. See Videos 1 and 2. n > 30, *P < 0.05, unpaired t test. (g) Time-lapse images of RPE-1 cells using a Nanolive microscope in LD-FBS without and with 5 µM myriocin for 20 h. The nuclear shape of the daughter cell is highlighted in the 60-min time point by a white line contour. Scale bar = 20 µm. See Videos 3 and 4.