Gene expression analysis in response to SPT inhibition. (a) Gene expression analysis of yeast cells treated with 5 µM myriocin for 0, 60, or 180 min grown in batch culture. Experiments (columns), genes (rows), and log2 ratios relative to untreated controls (time 0 min) are ordered by chromosome position. Upregulated genes are shown in yellow, downregulated genes are in blue, and genes that do not change are in black. (b) Percentage of transcripts that show a 1.4-fold change in levels relative to untreated controls in 0, 60, or 180 min. Relative levels of LCB, ceramides, and viability at each time point are shown below. Pearson’s correlation coefficient r is shown. (c) Hierarchical clustering of the gene expression data presented in Fig. 2 b.
