Synthesis of LCBs determines nuclear shape and volume in yeast. (a) De novo synthesis pathways of the three major classes of structural lipids in human and yeast cells are conserved. (b) De novo synthesis pathway of sphingolipids in yeast and human. DHS and PHS are the main LCB in yeast. DHS and Sph are the main LCB in human. (c) Strategies to target sphingolipid levels in yeast cells. (d) LCB and ceramide levels in yeast cells. Data are obtained from Hwang et al. (2019). Error bars represent the standard deviations (n = 3). *P < 1e-4, one-way ANOVA. NS, P > 0.05. (e) Relative doubling time is not affected upon deleting LCB4 or LAG1. Error bars represent standard deviations (n = 3). NS, P > 0.05, one-way ANOVA test.