Coilin exchange between CB and the nucleoplasm is determined by its total concentration. (A) FRAP analysis of coilin exchange in and out the CB. Four different recovery curves (each depicted with a distinct color) from cells expressing different levels of EGFP-coilin (indicated by a.u.) are presented, showcasing the high variability in recovery. (B) Correlation analysis between coilin expression and CB FRAP recovery plateau. Dots are single FRAP results. Dashed lines are logarithmic fit of the data and R² the coefficient of determination. (C) Correlation plot between coilin concentration measured in the nucleoplasm and the model prediction base on FRAP analysis of CB.