Global fitting of FRAP curves. (A–C) Individual FRAP curves in the nucleoplasm of (A) EGFP, (B) EGFP-coilin^1–136/R8A, and (C) EGFP-coilin^R8A+K496E were globally fitted with Eqs. 1, 2, and 3, respectively. The fitted parameters are in Tables S2.