Coilin self-interaction reduces coilin movement inside the nucleus. (A) Autocorrelation functions derived from spFCS performed in the nucleoplasm of HeLa coilin^KO cells expressing EGFP-coilin^WT(cyan), EGFP-coilin^K496E (yellow), EGFP-coilin^R8A (green), EGFP-coilin^R8A+K496E (purple), or EGFP (grey). (B) Dotplot graph obtained from the fitting of the autocorrelation functions. X axis represents the diffusion coefficient (in log scale), and Y axis represents the ratio of the two amplitudes. Rounded dots represent the results from single cells; square dots are the averages of the population. The diffusion coefficient of the fast component was fixed to be equal to the diffusion coefficient of EGFP. (C) Molecular brightness obtained from the spFCS measurements shown in B. The data represent the mean, and the error bars represent standard deviation, and the number of measurements per mutant are indicated by the number in the bar. (D and E) FRAP comparison of (D) EGFP-coilin^WT and EGFP-coilin^K496E and (E) EGFP-coilin^R8A and EGFP-coilin^R8A+K496E. FRAP of EGFP is reported for reference.