Characterization of coilin variants. (A) Overexpression of EGFP-coilin^R8A disrupts CBs. Hela^WT cells transfected with EGFP alone or EGFP-coilin^R8A (green). Coilin (red) and snRNPs (magenta) were immunolabeled. Cells with low and high expression of EGFP-coilin^R8A are shown. Scale bar represents 5 µm. (B) Schematic representation of coilin full-length and coilin-cleaved fragment (coilin^1–136). (C) Western blot of EGFP-coilin^WT (line 1) and EGFP-coilin^1–136 (line 2) using anti-GFP antibodies. (D) Transfection of HeLa^coilinKO and parental HeLa (HeLa WT) cells with coilin fragment containing the R8A mutation (EGFP-coilin^1–136/R8A) and WT version (EGFP-coilin^1–136). Scale bar represents 5 µm. (E) FRAP comparison of different coilin constructs with mutations/deletions in the C-terminal Tudor domain. (F) FRAP comparison of the truncated versions of coilin (EGFP-coilin^1–136 and EGFP-coilin^1–136/R8A) and EGFP. Source data are available for this figure: SourceData FS1.