Coilin movement is restricted across the CB border. (A) 2D-pCF analysis of a solution of fluorescence beads diffusing in water containing large agarose beads (AB). The first image displays the fluorescence signal. The second image represents the anisotropy level in the solution. The third image displays the preferential direction of movement of the fluorescence beads (where high anisotropy is detected). The fourth image is a model build based on the result of 2D-pCF. The agarose beads are labeled by AB. Fluorescence beads are drawn as green dots, and arrows indicate possible direction for free movement. (B) 2D-pCF analysis of Hela^coilinKO cells expressing EGFP-coilin^WT. The first image displays the fluorescence EGFP signal. The second image represents the anisotropy level in the same cell. A mask excluding the region outside the cell nucleus lacking a visible EGFP signal was drawn to exclude near-zero values produced by noise. The third image merges the fluorescent intensity and the preferential direction of movement of coilin, where high anisotropy is detected (white lines). The fourth image is a model build based on the result of 2D-pCF. Cytoplasm and nucleus side is indicated in the image. Monomeric and oligomeric coilin is drawn as small and big green dots. Arrows indicate possible direction for free movement. Orange rectangles represent nuclear pores whose positions were inferred from EGFP-coilin movement.