Subcompartmentalization and internal dynamics of coilin inside CBs. (A) STED (left) and SIM (right) image of a CB immunolabeled for coilin. Scale bar, 200 nm. (B) 12 consecutive single-plane frames of HeLa^coilin-EGFP stable cell line showing one CB. Time-lapse was recorded at 800 ms/frame. Color bar indicates the fluorescence intensity visualized by cool color map in ImageJ. Scale bar represents 500 nm. (C) FRAP performed in half (left) or whole (right) CB. Images display the CBs used for the graphs. Contrast was enhanced to facilitate readability of the images. Color bar indicates the fluorescence intensity visualized by cool color map in ImageJ. Scale bar represents 500 nm.